Canyoncreek,
Interesting information. One thing I did notice that I did not mention when separating 4 for the forage pond and 4 for the small stream is the absence of color on the top of the back by the dorsal fin on a couple of fish. “Females axe similar to each other, lacking high color.” I assumed these were females and I sorted accordingly. Another article I found talks about the lack of success breeding Spotfin shiners indoors and how outdoor ponds provided greater than 95% success. https://www.uwsp.edu/cols-ap/nadf/P...%20Spotfin%20Shiner-%20Fischer%20PPT.pdf
Thanks for posting the article!