We throw more than that into the bait well on the boat when we're heading out to set catfish lines on the MO.
That's maybe 15 gallons of water when full, and half of it will slosh out the overflow pipe while traveling.
They stay plenty frisky with the two-stone Power Bubbles running.

If I was transporting that many for relocation I'd want more water. 150qt cooler would do.
For sure keep it out of the Florida sun.