I'm going to add bacteria to bump it back up after this last treatment. How long should I wait to ensure the supplement has the best effect?

.10 surface acre pond, 10.5 foot deep. SW LA. The epitome of a mutt pond. BG, LMB, GSF, RES, BH, Warmouth, Longear Sunfish, Gambusia,Mud Minnows, Crappie, and now shiners!!...I subscribe!!