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Joined: Jan 2003
Posts: 293
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Joined: Jan 2003
Posts: 293 |
Hey thanks guys. Really great info. I do have a few follow-up questions Would it cost anything to get help from Texas A&M? Do they have a website or something where I might easily acess some of thier research? Do you know if there is anything else usefull to be learned from a DNA analysis, like how badly my bass genepool is inbred and in need of some new genetics? If so, could I tell from a single sample or would I need to sample a whole lot of bass?
Also, I'm home sick today with nothing to do so this morning I decided to try an extraction, but it didn't go as well as I had hoped. Everything went just fine until I added the alcohol. I could see the DNA precipitating and there was actualy more than I had expected but when I went to gather it up it didn't collect together in long, sticky fibers. It just floated around in little bitty chunks. I finally gave up trying to pick it up and just skimmed it off the alcohol with the suspended bits of DNA in it. I figured since you store it in alcohol anyways it couldn't be all that bad that I couldn't seperate them but I cannot figure out why the DNA wouldn't gather together. The best explaination that I can think of is either that it took too long to chop up and smash the fish meat into goo and having not used any meat tenderizer that enzymes dammaged the DNA in some way. Or more likely that the 91% Isopropyl Alcohol 9% water solution that I used was simply not pure enough. Have any of you experienced something like this before or have any idea what might have happened?
thanks -Scott
Take great care of it, or let someone else have it.
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