I believe 2 is a male also.

Are the suspect females getting a bulge in the area of the ovaries? My female bluegills are really popping out in that area.

Once they are in peek spawning condition you should be able to squeeze some milt out of the males. If you really want to be sure a capillary tube inserted a certain way and capped off on one end with your finger will produce some eggs when pulled out.

If pigs could fly bacon would be harder to come by and there would be a lot of damaged trees.