No prob... Mosquitofish are usually able to have several broods of babies before they need to mate again. Even if you don't have a male, your females may already have enough milt in them to produce several broods without mating again. Once they give birth, you'll have plenty of males for subsequent generations. Just a small 10 gallon tank with some cover in it and you'll have plenty of the little buggers in no time!

Males are usually much smaller and don't have that little black spot by the anal fin. Their anal fin is modified into a reproductive organ called a gonopodium... It's long and narrow, when in an aquarium its easy to see. Might help you see if you got a male and female or just two females.

Last edited by CJBS2003; 04/18/09 04:51 AM. Reason: added more info