Since you have the capability of checking DO levels at that depth, that you do a foot by foot analysis from top to bottom. I'm no expert, but I think your fish will find that layer of water that provides the nest of both temp and DO. Millions of years of evolution have conditioned all fish to seek out the best areas in their habitat. You can verify this with a depth/fish finder. The level where you see fish in the greatest numbers will be the prime depth.

.10 surface acre pond, 10.5 foot deep. SW LA. The epitome of a mutt pond. BG, LMB, GSF, RES, BH, Warmouth, Longear Sunfish, Gambusia,Mud Minnows, Crappie, and now shiners!!...I subscribe!!