I agree with you DrLuke, the sort of thing MNFISH2 is doing is fascinating to think about. There was a post last month about infusing dissolved oxygen into the column of a bottom diffuser that I found interesting that would seem to be applicable to this system as well, particularly if there is ice on the pond and the partial pressure of O2 can be increased between the ice and water surface. I have wondered if these systems of oxygenation/aeration could be tested in a standard swimming pool that is frozen. This would largely eliminate the biological variables when measuring DO at various depths. Obviously the information would not be directly applicable to a pond, but it might help to understand what is a reasonable expectation in a biological setting. As alluded to in the post from last month, could it be that DO can be increased in the water by pumping O2 under the ice and not letting it escape through a hole cut in the ice?