OK guys I have not posted much research lately. Burger how do you get me into these type questions.
I assume from this study that normal sex ratios in HSB are to be expected. If not then they would not need to sex reverse them and the normal mixed-sex yoy would not be 1 to 1 as indicated in the control groups.
Hormonal Sex Inversion in
Striped Bass and White Bass X Striped Bass Hybrids
Methods
The experiments were conducted at the Fish and
Aquaculture Research Station (Dor, Israel).
The young fish for the experiments were obtained from
kibbutz Hamaapil Fish Hatchery, where Morone
species are artificially propagated. Mixed-sex normal
progenies of striped bass and hybrid striped
bass (female white bass x male striped bass) were
used in the experiments. The androgen 17amethyltestosterone
(MT) was orally administrated
to fish with a dry prepared diet or with nauplii of
brine shrimp Artemia sp. at different periods after
hatching. Live food was used at the earlier stages
of fish ontogeny.
Experiment 1.—Three MT-treated groups and
one control group of the same origin were used in
this study. The 32-d-old white bass x striped bass
fry were collected from large production rearing
tanks (where the fish were fed rotifers) at kibbutz
Hamaapil Fish Hatchery and stocked into two
recirculating-water systems (460 fish/system) at
Dor Station. The fish in one system (group 1) were
fed with MT-enriched brine shrimp nauplii for 30
d from 34 to 64 d after hatching (d.a.h.), while the
fish stocked into the other system received intact
brine shrimp nauplii (control group; Table 1). Water
temperature was maintained at 21–228C. Later,
another batch of fish from the same hybrid progeny
was collected from the pond (where the fry were
stocked from production rearing tanks) and put
into two other recirculating-water systems (110
fish/system). The fish in one system were fed with
a dry diet containing MT for 30 d from 67 to 97
d.a.h. (experiment 1, group 2). The fish in the other
system (experiment 1, group 3) received regular
dry diet (no androgen added) up to the 97th d.a.h.
Afterwards, all fish surviving in this system (N 5
77) were transferred to the hormone-containing
diet for 30 d (from 98 to 128 d.a.h.; Table 1).Water
temperatures were 22–248C (67–97 d.a.h.) and 25–
26.58C (98–128 d.a.h.).
Experiment 2.—One MT-treatment group (group
1) and a corresponding control group taken from
another (than in experiment 1) hybrid progeny
were included. Fish were caught from a pond and
put into two recirculating-water systems (100 fish/
system). Group 1 received a diet containing MT
for 40 d (from 92 to 132 d.a.h.), while the control
group was fed a MT-free food (Table 1). Water
temperatures during the experimental period were
25–26.58C.
Fish Survival and Sex Ratio
The data on fish survival during the period of
hormonal treatment and sex distributions in experimental
and control groups are given in Table 1.
Experiment 1.—There was no significant difference
in fish survival between group 1 (29.3%) recieving
MT-enriched brine shrimp and the control
group (27.4%) fed intact nauplii during the same
period. Fish survival in experimental groups increased
with fish age and size up to 99% in group
3. The sex ratio in the control group did not differ
from 1:1 (P . 0.05). Groups 1 and 2 had similar
sex ratios (about 60% males) that did not differ
significantly from 1:1 (P . 0.05), but 12% of the
fish in group 1 had intersexual gonads. In group
3, 97.2% of fish were males (P , 0.001).
Experiment 2.—Fish survival was similar in
both groups. The sex ratio in the control group
was close to 1:1 (P . 0.05). In group 1, 95% of
the fish were males and the rest (5%) were intersex.