So IMO the question of "How do you know if its pure, did you have the genetics checked " on a lepomis hybrid is irrelevent. I bet not one person on this Forum has ever had or seen lepomis genes checked with Dave Willis being a possible exception. Now Green Carp (LMB) are a different matter.
Actually Eric there was a PHD at Illinois Natural History that was studying pumpkinseed bluegill hybrids in Canada. From my understanding he had the DNA for both hybrids and pure pumpkinseeds. I know this because I took in to mount a potential world record pumpkinseed or pumpkinseed hybrid. (It weighed 1 lb. 6 oz.)
The problem was, (I apologize if I posted this already on the site) it got complicated when I took it to a local biologist station to have it identified. One biologist came in late to work and said, "Hybrid!" from 20 feet away and refused to even look at it. The other was more conscientous and took the time to key it out down to the gillrakers. He believed it was a pure pumpkinseed even though it did not have the red tip to the ear tab. It was a dead ringer for a pumpkinseed, but just did not have the red tip to the ear tab.
Anyway, I was left with the dilemna that it now needed to be checked via DNA as these two biologists did not agree. The PHD at Illinois Natural History offered to check the DNA and but then apparently backed out when I wanted to arrange shipment of part of the carcass. That is, he wouldn't answer his emails and wouldn't return my calls.
So to this day we have no idea if it's a world record hybrid pumpkinseed (IGFA said they would make a category if it was), or it tied the world record as a pure pumpkinseed. My state does not even have a state record category for a pumpkinseed.
I'm still miffed at the arrogant biologist that refused to even look at it.