There is a powder on the market that removes ammonia and nitrites from the water, or locks them up so the fish aren't affected. Cecil told me about it, but I can't remember what it is. IIRC he bought it from Aquatic Ecosystems. You might want to get some for the trip.
Bill is right about monitoring temps. I moved 125 LMB last summer, 6"-8" fish. I moved them in bags, and put ice in the bottom of each 5 gal bucket according to the hatchery. Inside an air conditioned car. 3 hr trip and when I got there, ALL the LMB were on the bottom of the bags, mouths open and stiff. 25 fish per bag. I was able to save 100 of them, but lesson learned. 1) less fish per bag. 2) monitor temps 3)monitor fish once an hour.